Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody (GTX109939) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody (GTX109939) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody (GTX109939) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 μg of O-GlcNAc transferase antibody (GTX109939).
Western blot analysis was performed using O-GlcNAc transferase antibody (GTX109939).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
5% SDS-PAGE
O-GlcNAc transferase antibody (GTX109939) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal PLoS One in 2016. PMID: 27281159